Thy-1 expresses two signals for apical localization in epithelial cells. Academic Article uri icon

Overview

abstract

  • Recent work has shown that anchoring via glycosyl phosphatidylinositol (GPI) results in apical targeting for a variety of endogenous and transfected plasma membrane proteins expressed in epithelial cells. To further determine the correlation between GPI anchoring and apical localization, we expressed GPI-anchored and secretory forms of Thy-1 in Madin-Darby canine kidney cells by transfection. Native GPI-anchored Thy-1, normally expressed in thymocytes and neurons, was localized to the apical surface. A truncated form of Thy-1, lacking 22 out of 31 hydrophobic amino acids at the COOH-terminus, was also constructed; this deletion blocked attachment of the GPI anchor and resulted in apical secretion of Thy-1. In combination with previous results, our observations indicate that Thy-1 contains apical targeting information in its protein sequence as well as in the GPI anchor.

publication date

  • April 1, 1991

Research

keywords

  • Antigens, Surface
  • Cell Membrane
  • Signal Transduction

Identity

Scopus Document Identifier

  • 0025853784

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.1991.260.4.C715

PubMed ID

  • 1708202

Additional Document Info

volume

  • 260

issue

  • 4 Pt 1