Bortezomib, but not cisplatin, induces mitochondria-dependent apoptosis accompanied by up-regulation of noxa in the non-small cell lung cancer cell line NCI-H460. Academic Article uri icon

Overview

abstract

  • Defects in the apoptotic machinery may contribute to chemoresistance of non-small cell lung cancer (NSCLC) cells. We have previously showed a deficiency in mitochondria-dependent caspase-9 activation in NSCLC H460 cells after exposure to cisplatin, a drug widely used to treat NSCLC. Here we show that, unlike cisplatin, the novel anticancer agent bortezomib efficiently induces caspase-9 activation and apoptosis in H460 cells. A comparative analysis of molecular events underlying cell death in bortezomib-treated versus cisplatin-treated H460 cells revealed that bortezomib, but not cisplatin, caused a rapid and abundant release of cytochrome c and Smac/DIABLO from mitochondria. This was associated with a marked increase in levels of the BH3-only proapoptotic protein Noxa and the antiapoptotic protein Mcl-1. Taken together, our data show that bortezomib, by promoting a proapoptotic shift in the levels of proteins involved in mitochondrial outer-membrane permeabilization, is a potent activator of the mitochondrial pathway of apoptosis in NSCLC cells. Our preclinical results support further investigation of bortezomib-based therapies as a possible new treatment modality for NSCLC.

publication date

  • March 1, 2007

Research

keywords

  • Antineoplastic Agents
  • Apoptosis
  • Boronic Acids
  • Carcinoma, Non-Small-Cell Lung
  • Cisplatin
  • Lung Neoplasms
  • Mitochondria
  • Proto-Oncogene Proteins c-bcl-2
  • Pyrazines

Identity

Scopus Document Identifier

  • 34147157853

Digital Object Identifier (DOI)

  • 10.1158/1535-7163.MCT-06-0577

PubMed ID

  • 17363497

Additional Document Info

volume

  • 6

issue

  • 3