Isolation, growth, and purification of defective adenovirus deletion mutants. Academic Article uri icon

Overview

abstract

  • Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.

publication date

  • January 1, 2007

Research

keywords

  • Adenoviridae
  • Defective Viruses
  • Mutation

Identity

Scopus Document Identifier

  • 34547424259

Digital Object Identifier (DOI)

  • 10.1385/1-59745-166-5:19

PubMed ID

  • 17401161

Additional Document Info

volume

  • 130