Rab10, a target of the AS160 Rab GAP, is required for insulin-stimulated translocation of GLUT4 to the adipocyte plasma membrane. Academic Article uri icon

Overview

abstract

  • GLUT4 trafficking to the plasma membrane of muscle and fat cells is regulated by insulin. An important component of insulin-regulated GLUT4 distribution is the Akt substrate AS160 rab GTPase-activating protein. Here we show that Rab10 functions as a downstream target of AS160 in the insulin-signaling pathway that regulates GLUT4 translocation in adipocytes. Overexpression of a mutant of Rab10 defective for GTP hydrolysis increased GLUT4 on the surface of basal adipocytes. Rab10 knockdown resulted in an attenuation of insulin-induced GLUT4 redistribution to the plasma membrane and a concomitant 2-fold decrease in GLUT4 exocytosis rate. Re-expression of a wild-type Rab10 restored normal GLUT4 translocation. The basal increase in plasma-membrane GLUT4 due to AS160 knockdown was partially blocked by knocking down Rab10 in the same cells, further indicating that Rab10 is a target of AS160 and a positive regulator of GLUT4 trafficking to the cell surface upon insulin stimulation.

publication date

  • April 1, 2007

Research

keywords

  • Adipocytes
  • Cell Membrane
  • Glucose Transporter Type 4
  • Insulin
  • rab GTP-Binding Proteins

Identity

Scopus Document Identifier

  • 33947578085

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2007.03.001

PubMed ID

  • 17403373

Additional Document Info

volume

  • 5

issue

  • 4