Validation of the MycoAlign system for Mycobacterium spp. identification. Academic Article uri icon

Overview

abstract

  • Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.

publication date

  • June 15, 2007

Research

keywords

  • Bacterial Proteins
  • Chaperonins
  • Mycobacterium
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S

Identity

Scopus Document Identifier

  • 34548634191

Digital Object Identifier (DOI)

  • 10.1016/j.diagmicrobio.2007.04.012

PubMed ID

  • 17572036

Additional Document Info

volume

  • 59

issue

  • 1