Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts. Academic Article uri icon

Overview

abstract

  • BACKGROUND: We investigated the hypothesis that Foxp3+ cells are an integral component of antiallograft immunity but are dominated by pathogenic effectors. METHODS: Wild-type H-2b C57BL/6 (B6) mice or B6 mice with a targeted disruption of c-Rel gene (c-Rel-/-) were used as recipients of islet grafts from allogeneic DBA/2 (H-2d) mice or syngeneic B6 mice. We developed kinetic quantitative polymerase chain reaction assays and measured intragraft expression of mRNA for Foxp3, IDO, cytolytic molecules, proinflammatory cytokines, and chemokines/receptors. RESULTS: Intraislet levels of mRNA for Foxp3, IDO, CD3, CD25, tumor necrosis factor-alpha, RANTES, IP-10, and CXCR3 were highest in DBA/2 islet allografts from WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or syngeneic B6 islet grafts from WT B6 mice. The ratio of granzyme B or IFN-gamma to Foxp3 was higher with the DBA/2 islet allografts from the WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or B6 islet grafts from WT B6 recipients. CONCLUSIONS: Foxp3+ cells are an integral component of acute rejection of allografts but may be dominated by pathogenic effectors.

publication date

  • June 27, 2007

Research

keywords

  • Forkhead Transcription Factors
  • Graft Rejection
  • Indoleamine-Pyrrole 2,3,-Dioxygenase
  • Islets of Langerhans Transplantation

Identity

Scopus Document Identifier

  • 34250829022

Digital Object Identifier (DOI)

  • 10.1097/01.tp.0000263991.74052.46

PubMed ID

  • 17589350

Additional Document Info

volume

  • 83

issue

  • 12