Purified, proteolytically mature HIV type 1 SOSIP gp140 envelope trimers. Academic Article uri icon

Overview

abstract

  • HIV type 1 (HIV-1) envelope is a noncovalent trimer of gp120-gp41 heterodimers, and its lability has hindered structural studies. SOSIP gp140 is a soluble, proteolytically mature form of the HIV-1 envelope wherein gp120-gp41 interactions are stabilized via a disulfide bond and gp41 contains an additional trimer-stabilizing point mutation. We describe the isolation of a substantially pure preparation of SOSIP gp140 trimers derived from KNH1144, a subtype A isolate. Following initial purification, the only significant contaminant was higher-order gp140 aggregates; however, 0.05% Tween 20 quantitatively converted these aggregates into trimers. The surfactant effect was rapid, dose dependent, and similarly effective for a subtype B SOSIP gp140. Surfactant-treated SOSIP gp140 retained favorable antigenicity and formed compact trimers 12-13 nm in size as determined by electron microscopy. This report provides the first description of homogeneous, cleaved HIV-1 envelope trimers. These proteins may be useful as vaccine immunogens and for studying structure-function relationships within the HIV-1 envelope glycoproteins.

publication date

  • June 1, 2007

Research

keywords

  • Gene Products, env
  • HIV-1

Identity

Scopus Document Identifier

  • 34447282574

Digital Object Identifier (DOI)

  • 10.1089/aid.2006.0261

PubMed ID

  • 17604546

Additional Document Info

volume

  • 23

issue

  • 6