Connexin 43 confers resistance to hydrogen peroxide-mediated apoptosis. Academic Article uri icon

Overview

abstract

  • The current study aimed to understand the anti-apoptotic effect of overexpressed gap junction forming protein connexin (Cx) 43 in C6 glioma cells. C6 cells exposed to hydrogen peroxide (H2O2) or staurosporine demonstrated morphological and biochemical changes consistent with apoptosis, whereas C6 cells expressing Cx43 demonstrated relative resistance to H2O2, but not to staurosporine. This selective protection against H2O2 was due to inhibition of caspase-3 activation in Cx43 expressing cells. siRNA knockdown experiments in rat primary astrocytes confirmed the presence of endogenous Cx43-mediated anti-apoptotic effect. Cx43 interacts with the upstream apoptosis signal-regulating kinase 1 known to mediate H2O2-induced apoptosis providing a possible mechanism for protection. These findings provided new evidence for regulation of the mitogen activated protein kinase pathway and apoptosis by Cx43 implicating this protein in intracellular signaling beyond its role as a gap junction forming protein on the plasma membrane.

publication date

  • August 22, 2007

Research

keywords

  • Apoptosis
  • Brain
  • Brain Neoplasms
  • Connexin 43
  • Glioma
  • Hydrogen Peroxide

Identity

PubMed Central ID

  • PMC2001300

Scopus Document Identifier

  • 34548431646

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2007.08.066

PubMed ID

  • 17761141

Additional Document Info

volume

  • 362

issue

  • 3