The association of the activation-inducible tumor necrosis factor receptor and ligand with lumbar disc herniation. Academic Article uri icon

Overview

abstract

  • PURPOSE: Herniated nucleus pulposus fragments are recognized by the immune system as a foreign-body, which results in an autoimmune reaction. Human activation-inducible tumor necrosis factor receptor (AITR) and its ligand, AITRL, are important costimulatory molecules in the pathogenesis of autoimmune diseases. Despite the importance of these costimulatory molecules in autoimmune disease, their role in the autoimmune reaction to herniated disc fragments has yet to be explored. The purpose of the present study is to investigate whether the overexpression of AITR and AITRL might be associated with lumbar disc herniation. MATERIALS AND METHODS: The study population consisted of 20 symptomatic lumbar disc herniation patients. Ten macroscopically normal control discs were obtained from patients with spinal fractures managed with anterior procedures that involved a discectomy. Peripheral blood samples from both the study patients and controls were collected. The expression levels of AITR and AITRL were investigated by flow cytometric analysis, confocal laser scanning microscopy, immunohistochemistry and by reverse transcriptase-polymerase chain reaction (RT-PCR). The soluble AITR and AITRL serum levels were measured by an enzyme-linked immunosorbent assay. RESULTS: Flow cytometric analysis revealed significantly higher levels of both AITR and AITRL in the lumbar disc herniation patients than in the controls. The AITRL expression levels were also increased in patients with lumbar disc herniation, shown by using confocal laser scanning microscopy, immunohisto-chemistry, and RT-PCR. Finally, soluble AITR and AITRL were elevated in the patients with lumbar disc herniations. CONCLUSION: The AITR and AITRL are increased in both the herniated disc tissue and the peripheral blood of patients with lumbar disc herniation.

publication date

  • October 31, 2007

Research

keywords

  • Intervertebral Disc Displacement
  • Lumbar Vertebrae
  • Receptors, Nerve Growth Factor
  • Receptors, Tumor Necrosis Factor
  • Tumor Necrosis Factors

Identity

PubMed Central ID

  • PMC2628152

Scopus Document Identifier

  • 36248938590

Digital Object Identifier (DOI)

  • 10.3349/ymj.2007.48.5.839

PubMed ID

  • 17963343

Additional Document Info

volume

  • 48

issue

  • 5