An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue. Academic Article uri icon

Overview

abstract

  • Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors.

publication date

  • December 10, 2007

Research

keywords

  • Fibroblasts

Identity

PubMed Central ID

  • PMC2326106

Scopus Document Identifier

  • 41149104882

Digital Object Identifier (DOI)

  • 10.1369/jhc.7A7287.2007

PubMed ID

  • 18071065

Additional Document Info

volume

  • 56

issue

  • 4