Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae. Academic Article uri icon

Overview

abstract

  • This unit provides an overview of centrifugation-based fractionation procedures adapted for the yeast Saccharomyces cerevisiae. The goals, merits, limitations, and critical parameters of are discussed in order to facilitate the development of subcellular fractionation strategies. Topics include yeast cell lysate preparation, differential velocity centrifugation, density gradient centrifugation, and the analysis of subcellular fractions.

publication date

  • May 1, 2001

Research

keywords

  • Cell Fractionation
  • Centrifugation
  • Mycology
  • Saccharomyces cerevisiae

Identity

Scopus Document Identifier

  • 40949101672

Digital Object Identifier (DOI)

  • 10.1002/0471143030.cb0307s07

PubMed ID

  • 18228359

Additional Document Info

volume

  • Chapter 3