Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells. Academic Article uri icon

Overview

abstract

  • While all known microbial superantigens are mitogenic for human peripheral blood lymphocytes (PBL), the functional response induced by Mycoplasma arthritidis-derived superantigen (MAM) is unique in that MAM stimulation of PBL consistently results in T cell-dependent B cell activation characterized by polyclonal IgM and IgG production. These immunostimulatory effects of MAM on the humoral arm of the human immune system warranted a more precise characterization of MAM-reactive human T cells. Using an uncloned MAM reactive human T cell line as immunogen, we have generated a monoclonal antibody (mAb) (termed C1) specific for the T cell receptor V beta gene expressed by the major fraction of MAM-reactive human T cells, V beta 17. In addition, a V beta 17- MAM-reactive T cell population exists, assessed by MAM, induced T cell proliferation and cytotoxic T cell activity. mAb C1 will be useful in characterizing the functional properties of V beta 17+ T cells and their potential role in autoimmune disease.

publication date

  • October 1, 1991

Research

keywords

  • Antibodies, Monoclonal
  • Mitogens
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes
  • T-Lymphocytes, Helper-Inducer

Identity

PubMed Central ID

  • PMC2118960

Scopus Document Identifier

  • 0026048460

Digital Object Identifier (DOI)

  • 10.1084/jem.174.4.891

PubMed ID

  • 1833503

Additional Document Info

volume

  • 174

issue

  • 4