Suspended-drop electroporation for high-throughput delivery of biomolecules into cells. Academic Article uri icon

Overview

abstract

  • We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.

publication date

  • April 13, 2008

Research

keywords

  • Electroporation
  • Transfection

Identity

PubMed Central ID

  • PMC2668720

Scopus Document Identifier

  • 42949145404

Digital Object Identifier (DOI)

  • 10.1038/nmeth.1201

PubMed ID

  • 18408727

Additional Document Info

volume

  • 5

issue

  • 5