5' regulatory region of a novel cytokine gene mediates selective activation by interferon gamma. Academic Article uri icon

Overview

abstract

  • A newly described member of the platelet factor 4 family of cytokine genes, mig, is selectively induced by interferon gamma (IFN-gamma), and not IFN-alpha, in the mouse macrophage-like cell line RAW 264.7. Treatment of RAW 264.7 cells with IFN-gamma activated mig gene transcription as determined by nuclear run-on assays. mig genomic clones were isolated, and constructs containing genomic fragments that included the mig promoter region and the CAT reporter gene were prepared. In RAW 264.7 cells transfected with these constructs, CAT activity was found to be selectively induced by IFN-gamma. A 278-bp genomic fragment containing 235 nucleotides 5' of the transcription start site was sufficient for IFN-gamma-selective induction of CAT activity. Analysis of 5' deletion mutants localized a region essential for activation by IFN-gamma to within 64 nucleotides extending from -235 to -172. A genomic fragment containing this sequence was capable of conferring IFN-gamma inducibility to constructs with a heterologous promoter.

publication date

  • February 1, 1991

Research

keywords

  • Cytokines
  • Gene Expression Regulation
  • Interferon-gamma
  • Transcription, Genetic

Identity

PubMed Central ID

  • PMC2118797

Scopus Document Identifier

  • 0026013238

Digital Object Identifier (DOI)

  • 10.1084/jem.173.2.417

PubMed ID

  • 1899103

Additional Document Info

volume

  • 173

issue

  • 2