Mutational analysis of the human p53 gene in malignant melanoma. Academic Article uri icon

Overview

abstract

  • Nine metastatic melanoma cell lines and two melanocyte cell lines were analyzed for point mutations in highly conserved regions of the p53 gene. No mutations were detected in the two melanocytic cell lines and in eight melanoma cell lines. However, a C----T transition at codon 248, resulting in a substitution of tryptophan for arginine, was found in one melanoma cell line. On immunohistochemical staining, only this cell line showed reactivity for mouse monoclonal antibody 1801, which is immunoreactive with human p53 protein. The original paraffin-embedded specimen from which this mutant cell line was established was obtained, and sequence analysis detected the identical mutation in the p53 gene as that seen in the derived cell line. This is the first report indicating point mutations in the p53 gene in malignant melanocytic tissues.

publication date

  • February 1, 1991

Research

keywords

  • Exons
  • Genes, p53
  • Melanoma

Identity

Scopus Document Identifier

  • 0026110727

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0749.1991.tb00311.x

PubMed ID

  • 1924172

Additional Document Info

volume

  • 4

issue

  • 1