Allergen profiles of dog hair and dander, body fluids and tissues as defined by immunoblotting. Academic Article uri icon

Overview

abstract

  • The sera from 25 patients with clinical type I allergy against dogs were investigated by means of immunoblotting, using extracts of dog hair/dander, skin, hair, saliva, salivary gland, serum and liver. 96% of the patients' sera showed IgE antibodies reactive with 19- and 23-kilodalton (kDa) proteins in the hair/dander extract. The 23-kDa IgE-binding protein was preferentially detected in the hair extract and saliva but not in skin, salivary gland, serum and liver extracts. The 19-kDa band was strongly expressed in skin, but not in hair, serum and liver. Inhibition experiments using the 23-kDa containing extract prepared from hair and the 19-kDa containing extract prepared from skin revealed that these two proteins are likely to be immunologically independent allergens.

publication date

  • January 1, 1991

Research

keywords

  • Allergens
  • Body Fluids
  • Dogs
  • Hair
  • Prostatic Secretory Proteins

Identity

Scopus Document Identifier

  • 0026075076

Digital Object Identifier (DOI)

  • 10.1159/000235399

PubMed ID

  • 1937897

Additional Document Info

volume

  • 94

issue

  • 1-4