Interleukin-10-secreting T cells define a suppressive subset within the HIV-1-specific T-cell population. Academic Article uri icon

Overview

abstract

  • Recent studies have indicated that Treg contribute to the HIV type 1 (HIV-1)-related immune pathogenesis. However, it is not clear whether T cells with suppressive properties reside within the HIV-1-specific T-cell population. Here, PBMC from HIV-1-infected individuals were stimulated with a 15-mer Gag peptide pool, and HIV-1-specific T cells were enriched by virtue of their secretion of IL-10 or IFN-gamma using immunomagnetic cell-sorting. Neither the IL-10-secreting cells nor the IFN-gamma-secreting cells expressed the Treg marker FOXP3, yet the IL-10-secreting cells potently suppressed anti-CD3/CD28-induced CD4(+) as well as CD8(+) T-cell proliferative responses. As shown by intracellular cytokine staining, IL-10- and IFN-gamma-producing T cells represent distinct subsets of the HIV-1-specific T cells. Our data collectively suggest that functionally defined HIV-1-specific T-cell subsets harbor potent immunoregulatory properties that may contribute to HIV-1-associated T-cell dysfunction.

publication date

  • May 1, 2009

Research

keywords

  • HIV Infections
  • HIV-1
  • Interleukin-10
  • T-Lymphocyte Subsets
  • T-Lymphocytes, Regulatory

Identity

PubMed Central ID

  • PMC2742768

Scopus Document Identifier

  • 67649991445

Digital Object Identifier (DOI)

  • 10.1002/eji.200839002

PubMed ID

  • 19384871

Additional Document Info

volume

  • 39

issue

  • 5