Quantification and calibration of images in fluorescence microscopy. Academic Article uri icon

Overview

abstract

  • Fluorescence microscopy is a method widely used in life sciences to image biological processes in living and fixed cells or in fixed tissues. Quantification and calibration of images in fluorescence microscopy is notoriously difficult. We have developed a new methodology to prepare tissue "phantoms" that contain known amounts of (i) fluorophore, (ii) DNA, (iii) proteins, and (iv) DNA oligonucleotide standards. The basis of the phantoms is the ability of gelatin to act as a matrix for the conjugation of fluorophores as either a free-flowing liquid or a gelatinous solid depending on temperature (> or = 40 and < or = 4 degrees C).

publication date

  • June 1, 2010

Research

keywords

  • Microscopy, Fluorescence

Identity

Scopus Document Identifier

  • 77954460238

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2010.05.029

PubMed ID

  • 20513437

Additional Document Info

volume

  • 404

issue

  • 2