Mapping catalytically important regions of an enzyme using two-codon insertion mutagenesis: a case study correlating beta-lactamase mutants with the three-dimensional structure. Academic Article uri icon

Overview

abstract

  • Two-codon insertion mutants throughout the beta-lactamase (Bla)-encoding gene were characterized. Second site revertants of various mutants were isolated, mapped and sequenced. The activity of the mutants and the ability to get revertants showed a positive correlation with increasing distance from the active site, based on the three-dimensional structure of Bla. This observation is discussed as it may pertain to the generalized use of two-codon insertion mutagenesis in mapping important catalytic regions in enzymes.

publication date

  • April 1, 1991

Research

keywords

  • Mutagenesis, Insertional
  • beta-Lactamases

Identity

Scopus Document Identifier

  • 0025730653

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(91)90349-g

PubMed ID

  • 2055479

Additional Document Info

volume

  • 100