Dicer is a key player in oocyte maturation. Academic Article uri icon

Overview

abstract

  • OBJECTIVE: Apply Dicer siRNA to study functions of Dicer and miRNA during oogenesis. MATERIALS AND METHODS: Mouse oocytes were injected with Dicer siRNA and negative control siRNA and then matured in vitro. After IVM, oocytes were examined for maturation rates, spindle and chromosomal organization, and various gene expressions. RESULTS: Dicer siRNA significantly reduced maturation rates, increased abnormal spindle and chromosomal organization, and reduced the transcripts of Dicer miRNAs, spindle formation proteins (plk1 and AURKA) and spindle check points (Bub1, Bublb). Depletion of bulb16 markedly prohibited the first polar body extrusion and increased the incidence of misaligned chromosomes and abnormal meiotic spindle assembly. CONCLUSION: Dicer siRNA triggered a cascade reduction for gene expressions starting from Dicer to miRNAs than to spindle assembly proteins and checkpoints which led to abnormal spindle and chromosomal organization. Thus, Dicer and miRNA appeared to play an important role during oogenesis and were essential for meiotic completion.

publication date

  • September 9, 2010

Research

keywords

  • DEAD-box RNA Helicases
  • Endoribonucleases
  • Meiosis
  • Oogenesis

Identity

PubMed Central ID

  • PMC2965346

Scopus Document Identifier

  • 78349256591

Digital Object Identifier (DOI)

  • 10.1126/science.1175326

PubMed ID

  • 20827505

Additional Document Info

volume

  • 27

issue

  • 9-10