Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae. Academic Article uri icon

Overview

abstract

  • Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone 3beta-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols.

publication date

  • September 1, 2010

Research

keywords

  • Flavones
  • Flavonols
  • Industrial Microbiology
  • Streptomyces

Identity

Scopus Document Identifier

  • 77957741042

Digital Object Identifier (DOI)

  • 10.4014/jmb.1005.05038

PubMed ID

  • 20890094

Additional Document Info

volume

  • 20

issue

  • 9