Discovery of recurrent t(6;7)(p25.3;q32.3) translocations in ALK-negative anaplastic large cell lymphomas by massively parallel genomic sequencing. Academic Article uri icon

Overview

abstract

  • The genetics of peripheral T-cell lymphomas are poorly understood. The most well-characterized abnormalities are translocations involving ALK, occurring in approximately half of anaplastic large cell lymphomas (ALCLs). To gain insight into the genetics of ALCLs lacking ALK translocations, we combined mate-pair DNA library construction, massively parallel ("Next Generation") sequencing, and a novel bioinformatic algorithm. We identified a balanced translocation disrupting the DUSP22 phosphatase gene on 6p25.3 and adjoining the FRA7H fragile site on 7q32.3 in a systemic ALK-negative ALCL. Using fluorescence in situ hybridization, we demonstrated that the t(6;7)(p25.3;q32.3) was recurrent in ALK-negative ALCLs. Furthermore, t(6;7)(p25.3;q32.3) was associated with down-regulation of DUSP22 and up-regulation of MIR29 microRNAs on 7q32.3. These findings represent the first recurrent translocation reported in ALK-negative ALCL and highlight the utility of massively parallel genomic sequencing to discover novel translocations in lymphoma and other cancers.

publication date

  • October 28, 2010

Research

keywords

  • Chromosomes, Human, Pair 6
  • Chromosomes, Human, Pair 7
  • Lymphoma, Large-Cell, Anaplastic
  • Translocation, Genetic

Identity

PubMed Central ID

  • PMC3035081

Scopus Document Identifier

  • 78751692894

Digital Object Identifier (DOI)

  • 10.1038/modpathol.2010.225

PubMed ID

  • 21030553

Additional Document Info

volume

  • 117

issue

  • 3