Cytokine production in a model of wound healing: the appearance of MIP-1, MIP-2, cachectin/TNF and IL-1. Academic Article uri icon

Overview

abstract

  • Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.

authors

  • Fahey, Thomas J
  • Sherry, Barbara
  • Tracey, K J
  • van Deventer, Sander
  • Jones, W G
  • Minei, J P
  • Morgello, Susan
  • Shires, G T
  • Cerami, Anthony

publication date

  • March 1, 1990

Research

keywords

  • Cytokines
  • Interleukin-1
  • Leukocytes
  • Monokines
  • Tumor Necrosis Factor-alpha
  • Wound Healing

Identity

Scopus Document Identifier

  • 0025400674

Digital Object Identifier (DOI)

  • 10.1016/1043-4666(90)90002-b

PubMed ID

  • 2104219

Additional Document Info

volume

  • 2

issue

  • 2