Glutathione depletion and cytotoxicity of buthionine sulphoximine and SR2508 in rodent and human cells. Academic Article uri icon

Overview

abstract

  • SR2508 (1 mM) increases the rate of glutathione (GSH) depletion by L-buthionine-S-R-sulphoximine (BSO) in hypoxic V79 rodent and A549 human cells. Specifically, the GSH content for V79 and A549 cells, after incubating for about 6 hr with 50 and 100 microM BSO, respectively, was lower by at least 10-fold when 1 mM SR2508 was present. In addition, 1 mM SR2508 is extremely toxic to hypoxic cells with lower GSH content. Survival probabilities of GSH-depleted V79 and A549 cells are about 10(-3) after 10 hr incubation with 1 mM SR2508. By itself, 1 mM SR2508 or 50-100 microM BSO decreased cellular viability by about 50% with a 10 hr treatment period. Both the phenomena described above are preferential towards hypoxic cells with minimal effect on aerobic cells.

publication date

  • February 1, 1990

Research

keywords

  • Antimetabolites
  • Cell Survival
  • Glutathione
  • Methionine Sulfoximine
  • Nitroimidazoles
  • Radiation-Sensitizing Agents

Identity

Scopus Document Identifier

  • 0025219282

Digital Object Identifier (DOI)

  • 10.1016/0360-3016(90)90097-4

PubMed ID

  • 2137437

Additional Document Info

volume

  • 18

issue

  • 2