Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma. Academic Article uri icon

Overview

abstract

  • Anaplastic large-cell lymphomas (ALCLs) are a group of clinically and biologically heterogeneous diseases including the ALK(+) and ALK(-) systemic forms. Whereas ALK(+) ALCLs are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK(-) ALCL are missing, and their distinction from other T-cell non-Hodgkin lymphomas (T-NHLs) remains controversial. In the present study, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable of recognizing ALK(-) ALCL. Application of quantitative RT-PCR in independent datasets from cryopreserved and formalin-fixed paraffin-embedded samples validated a 3-gene model (TNFRSF8, BATF3, and TMOD1) able to successfully separate ALK(-) ALCL from peripheral T-cell lymphoma not otherwise specified, with overall accuracy near 97%. In conclusion, our data justify the possibility of translating quantitative RT-PCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols.

publication date

  • June 27, 2012

Research

keywords

  • Biomarkers, Tumor
  • Genes, Neoplasm
  • Lymphoma, Large-Cell, Anaplastic
  • Molecular Diagnostic Techniques
  • Receptor Protein-Tyrosine Kinases

Identity

Scopus Document Identifier

  • 84865176067

Digital Object Identifier (DOI)

  • 10.1182/blood-2012-01-405555

PubMed ID

  • 22740451

Additional Document Info

volume

  • 120

issue

  • 6