The sperm penetration assay for the assessment of fertilization capacity. Academic Article uri icon

Overview

abstract

  • The sperm penetration assay, or zona-free hamster oocyte penetration assay, is utilized to measure the ability of sperm to undergo capacitation, fuse with the egg membrane, and decondense the sperm head within the cytoplasm of the oocyte, resulting in the formation of the male pronucleus. The test is scored by calculation of the percentage of ova that are penetrated (the original assay developed) or the average number of sperm penetrations per ovum (the sperm capacitation index of the optimized assay). The sperm penetration assay identifies those couples that will have a high likelihood of success with in vitro fertilization.

publication date

  • January 1, 2013

Research

keywords

  • Fertility
  • Semen Analysis
  • Sperm-Ovum Interactions
  • Spermatozoa

Identity

PubMed Central ID

  • PMC3633452

Scopus Document Identifier

  • 84934438128

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-038-0_10

PubMed ID

  • 22992907

Additional Document Info

volume

  • 927