Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography. Academic Article uri icon

Overview

abstract

  • A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C18 stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.

publication date

  • February 1, 1990

Research

keywords

  • Carbohydrates
  • Glycoproteins

Identity

Scopus Document Identifier

  • 0025295163

Digital Object Identifier (DOI)

  • 10.1007/BF01024981

PubMed ID

  • 2340075

Additional Document Info

volume

  • 9

issue

  • 1