Synthesis, self-assembling and gene delivery potential of a novel highly unsaturated, conjugated cationic phospholipid. Academic Article uri icon

Overview

abstract

  • The synthesis and self-assembling properties of a model compound in a new class of cationic phospholipids with a highly unsaturated conjugated fatty acid are described. In addition, the potential of this new lipid as a nucleic acid carrier was evaluated through lipoplex formulations employing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as helper lipid with and without the polycationic peptide protamine, together with a plasmid DNA (pDNA). Lipoplexes composed of this novel unsaturated lipid exhibited pDNA binding and protection from DNase I degradation when formulated with protamine. The new cationic lipid revealed transfection efficiency comparable to the commercial reference 1,2-dimyristoyl-sn-glycero-3-ethylphophocholine (EPC) in Chinese hamster ovary-K1 (CHO-K1) cells and performed equally to the standard reference Lipofectamine 2000 when the formulation included protamine.

publication date

  • April 6, 2013

Research

keywords

  • Gene Transfer Techniques
  • Phospholipids

Identity

Scopus Document Identifier

  • 84877061495

Digital Object Identifier (DOI)

  • 10.1016/j.chemphyslip.2013.03.002

PubMed ID

  • 23570750

Additional Document Info

volume

  • 170-171