Effects of cellular activation on anti-HIV-1 restriction factor expression profile in primary cells. Academic Article uri icon

Overview

abstract

  • Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.

publication date

  • August 21, 2013

Research

keywords

  • Gene Expression Profiling
  • HIV-1
  • Immunity, Innate
  • Leukocytes, Mononuclear

Identity

PubMed Central ID

  • PMC3807358

Scopus Document Identifier

  • 84886889387

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0009081

PubMed ID

  • 23966394

Additional Document Info

volume

  • 87

issue

  • 21