Intracellular locations of replication proteins and the origin of replication during chromosome duplication in the slowly growing human pathogen Helicobacter pylori. Academic Article uri icon

Overview

abstract

  • We followed the position of the replication complex in the pathogenic bacterium Helicobacter pylori using antibodies raised against the single-stranded DNA binding protein (HpSSB) and the replicative helicase (HpDnaB). The position of the replication origin, oriC, was also localized in growing cells by fluorescence in situ hybridization (FISH) with fluorescence-labeled DNA sequences adjacent to the origin. The replisome assembled at oriC near one of the cell poles, and the two forks moved together toward the cell center as replication progressed in the growing cell. Termination and resolution of the forks occurred near midcell, on one side of the septal membrane. The duplicated copies of oriC did not separate until late in elongation, when the daughter chromosomes segregated into bilobed nucleoids, suggesting sister chromatid cohesion at or near the oriC region. Components of the replication machinery, viz., HpDnaB and HpDnaG (DNA primase), were found associated with the cell membrane. A model for the assembly and location of the H. pylori replication machinery during chromosomal duplication is presented.

publication date

  • December 20, 2013

Research

keywords

  • Bacterial Proteins
  • DNA Replication
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Helicobacter pylori
  • Protein Transport

Identity

PubMed Central ID

  • PMC3957694

Scopus Document Identifier

  • 84893740116

Digital Object Identifier (DOI)

  • 10.1016/j.mib.2005.02.006

PubMed ID

  • 24363345

Additional Document Info

volume

  • 196

issue

  • 5