Identification of transcripts encoding a parathyroid hormone-like peptide in messenger RNAs from a variety of human and animal tumors associated with humoral hypercalcemia of malignancy. Academic Article uri icon

Overview

abstract

  • The syndrome of humoral hypercalcemia of malignancy (HHM) appears to be mediated in many instances by a parathyroid hormone-like peptide, which has recently been purified, sequenced, and cloned. Using a probe representing the coding region of the human PTH-like peptide, we examined by Northern analysis poly (A)+ RNA from a variety of human and animal tumors associated with HHM. Hybridizing transcripts were identified in mRNA from each of 12 human and each of four animal HHM-associated tumors, with a complex hybridization pattern observed in the human mRNAs and a relatively simple pattern observed in the animal mRNAs. Poly (A)+ RNA prepared from tumors of similar histological types unassociated with HHM failed to hybridize with the probe. Messenger RNA-dependent biological activity from the animal tumors was entirely eliminated in a hybridization-arrest experiment using a complementary oligonucleotide spanning the region of homology between human PTH and the PTH-like peptide. These findings indicate that the PTH-like peptide is associated with the syndrome of HHM in a wide spectrum of tumor types from a variety of mammalian species and that the PTH-like sequence in the proximal amino terminus of the peptide is highly conserved.

publication date

  • June 1, 1988

Research

keywords

  • Hypercalcemia
  • Neoplasm Proteins
  • Paraneoplastic Syndromes
  • Parathyroid Hormone
  • RNA, Messenger

Identity

PubMed Central ID

  • PMC442656

Scopus Document Identifier

  • 0023879546

Digital Object Identifier (DOI)

  • 10.1172/JCI113551

PubMed ID

  • 2454953

Additional Document Info

volume

  • 81

issue

  • 6