Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)). Academic Article uri icon

Overview

abstract

  • As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with hPNPase(old-35) expression, were identified. The majority of these genes were associated with cell communication, cell cycle, and chromosomal organization gene ontology categories. For a number of these genes, the positive or negative correlations with hPNPase(old-35) expression were consistent with transcriptional data extracted from the TCGA (The Cancer Genome Atlas) expression datasets for colon adenocarcinoma (COAD), skin cutaneous melanoma (SKCM), ovarian serous cyst adenocarcinoma (OV), and prostate adenocarcinoma (PRAD). Further analysis comparing the gene expression changes between Ad.hPNPase(old-35) infected HO-1 melanoma cells and HeLa cells overexpressing hPNPase(old-35) under the control of a doxycycline-inducible promoter, revealed global changes in genes involved in cell cycle and mitosis. Overall, this study provides further evidence that hPNPase(old-35) is associated with global changes in cell cycle-associated genes and identifies potential gene targets for future investigation.

publication date

  • December 1, 2014

Research

keywords

  • Cell Cycle
  • Exoribonucleases
  • Gene Expression Regulation, Neoplastic
  • Melanoma

Identity

PubMed Central ID

  • PMC4149605

Scopus Document Identifier

  • 84906708176

Digital Object Identifier (DOI)

  • 10.1038/srep02652

PubMed ID

  • 24729470

Additional Document Info

volume

  • 229

issue

  • 12