Plasma membrane protein polarity and trafficking in RPE cells: past, present and future. Review uri icon

Overview

abstract

  • The retinal pigment epithelium (RPE) comprises a monolayer of polarized pigmented epithelial cells that is strategically interposed between the neural retina and the fenestrated choroid capillaries. The RPE performs a variety of vectorial transport functions (water, ions, metabolites, nutrients and waste products) that regulate the composition of the subretinal space and support the functions of photoreceptors (PRs) and other cells in the neural retina. To this end, RPE cells display a polarized distribution of channels, transporters and receptors in their plasma membrane (PM) that is remarkably different from that found in conventional extra-ocular epithelia, e.g. intestine, kidney, and gall bladder. This characteristic PM protein polarity of RPE cells depends on the interplay of sorting signals in the RPE PM proteins and sorting mechanisms and biosynthetic/recycling trafficking routes in the RPE cell. Although considerable progress has been made in our understanding of the RPE trafficking machinery, most available data have been obtained from immortalized RPE cell lines that only partially maintain the RPE phenotype and by extrapolation of data obtained in the prototype Madin-Darby Canine Kidney (MDCK) cell line. The increasing availability of RPE cell cultures that more closely resemble the RPE in vivo together with the advent of advanced live imaging microscopy techniques provides a platform and an opportunity to rapidly expand our understanding of how polarized protein trafficking contributes to RPE PM polarity.

publication date

  • September 1, 2014

Research

keywords

  • Cell Membrane
  • Cell Polarity
  • Membrane Proteins
  • Retinal Pigment Epithelium

Identity

PubMed Central ID

  • PMC4502961

Scopus Document Identifier

  • 84906510164

Digital Object Identifier (DOI)

  • 10.1016/j.exer.2014.04.021

PubMed ID

  • 25152359

Additional Document Info

volume

  • 126