Isolation and culture of HCO3- -secreting intercalated cells. Academic Article uri icon

Overview

abstract

  • Intercalated cells of the distal nephron secrete either H+ or HCO3-. We have succeeded in isolating HCO3- -secreting intercalated cells from the rabbit kidney. When seeded onto collagen-coated permeable supports, these cells form monolayers with a resistance of 595 +/- 75 omega.cm2. The monolayers maintain the characteristics of an epithelium, with apical microvillae and tight junctions. They display the same polarity as do HCO3- -secreting intercalated cells in vivo, namely apical peanut lectin binding and apical Cl- -HCO3- exchange. The monolayers are capable of transepithelial HCO3- transport via this exchanger. The rate of Cl- -dependent transepithelial HCO3- transport is 4 +/- 0.4 nmol.min-1.cm-2. Transepithelial HCO3- transport is completely abolished by 50 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid applied to the apical side of the monolayer. These cultured HCO3- -secreting intercalated cells should prove useful for defining the cellular regulation of HCO3- secretion.

publication date

  • May 1, 1989

Research

keywords

  • Bicarbonates
  • Cell Separation
  • Kidney Tubules
  • Kidney Tubules, Collecting

Identity

Scopus Document Identifier

  • 0024591669

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.1989.256.5.C1004

PubMed ID

  • 2541617

Additional Document Info

volume

  • 256

issue

  • 5 Pt 1