Imaging of mitochondrial dynamics in motor and sensory axons of living mice. Academic Article uri icon

Overview

abstract

  • Appropriate distribution and supply of mitochondria to critical neuronal sites are thought to be necessary for the normal maintenance of neuronal architecture and activity, including synaptic plasticity and function. Imaging of neurons in vitro has provided understanding of the basic mechanisms of mitochondrial transport and the regulation of mitochondrial dynamics. However, in vivo imaging studies of neurons are preferable to in vitro approaches because of the advantage of being performed in their natural environment. Here, we present useful protocols to image and study axonal transport of mitochondria in vivo, in the peripheral nerves of mice. Imaging in motor and sensory axons of living mice allows researchers to analyze mitochondrial dynamics in two distinct neuronal populations that are often affected in peripheral neuropathies.

publication date

  • January 1, 2014

Research

keywords

  • Axons
  • Mice, Transgenic
  • Mitochondrial Dynamics
  • Motor Neurons
  • Sensory Receptor Cells

Identity

Scopus Document Identifier

  • 84925423608

Digital Object Identifier (DOI)

  • 10.1016/B978-0-12-801415-8.00006-0

PubMed ID

  • 25416354

Additional Document Info

volume

  • 547