High-performance liquid chromatographic analysis of acylated lipids containing pyrene fatty acids. Academic Article uri icon

Overview

abstract

  • A high-performance liquid chromatographic method for the separation and quantification of acylated lipids containing pyrene fatty acids is described. The method is adapted from a procedure originally developed for the analysis of tissue lipids (Christie, W. W. (1985) J. Lipid Res. 26, 507-512). Pyrenyl lipid analogs ranging in polarity from cholesteryl ester to lysophosphatidylcholine are completely resolved on a silica column in 50 min by gradient elution with a ternary solvent system. Furthermore, pyrene-labeled triglycerides are resolved according to the number of pyrene fatty acid residues incorporated. Pyrenyl lipids are detected at levels of 10(-13) mol by high-sensitivity fluorescence detection. Accurate quantification of pyrenyl lipids is obtained by correcting peak areas for mobile-phase quenching effects. The close correspondence between chromatograms obtained for the separation of labeled lipids extracted from Hep-G2 cells incubated with either 12-(1-pyrenyl)dodecanoic acid (fluorescence detection) or [1-14C]oleic acid (radioactivity detection) indicates that this HPLC method is equally suitable for analysis of native lipids.

publication date

  • April 1, 1989

Research

keywords

  • Chromatography, High Pressure Liquid
  • Fatty Acids
  • Lipids
  • Pyrenes

Identity

Scopus Document Identifier

  • 0024502115

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(89)90374-6

PubMed ID

  • 2543234

Additional Document Info

volume

  • 178

issue

  • 1