Aggregate and Microcarrier Cultures of Human Pluripotent Stem Cells in Stirred-Suspension Systems. Academic Article uri icon

Overview

abstract

  • Pluripotent stem cells can differentiate to any cell type and contribute to damaged tissue repair and organ function reconstitution. The scalable culture of pluripotent stem cells is essential to furthering the use of stem cell products in a wide gamut of applications such as screening of candidate drugs and cell replacement therapies. Human stem cell cultivation in stirred-suspension vessels enables the expansion of stem cells and the generation of differentiated progeny in quantities suitable for use in animal models and clinical studies. We describe methods of culturing human pluripotent stem cells in spinner flasks either as aggregates or on microcarriers. Techniques for assessing the quality of the culture and characterizing the cells based on the presentation of pertinent markers are also presented. Spinner flask culture with its relatively low capital and operating costs is appealing to laboratories interested in scaling up their production of stem/progenitor cells.

publication date

  • January 1, 2016

Research

keywords

  • Cell Culture Techniques
  • Pluripotent Stem Cells

Identity

PubMed Central ID

  • PMC5642038

Scopus Document Identifier

  • 84990203823

Digital Object Identifier (DOI)

  • 10.1007/7651_2015_312

PubMed ID

  • 26659793

Additional Document Info

volume

  • 1502