Near-Infrared Intraoperative Chemiluminescence Imaging. Academic Article uri icon

Overview

abstract

  • Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near-infrared light upon exposure to an aqueous solution of Ce(4+) in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9 pmol cm(-2) of the transition-metal-based ICI agent. As a proof of concept, we use ICI for the in vivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal-to-noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies.

publication date

  • July 29, 2016

Research

keywords

  • Fluorescent Dyes
  • Infrared Rays
  • Luminescence
  • Luminescent Measurements
  • Lymph Nodes
  • Organometallic Compounds

Identity

PubMed Central ID

  • PMC5061038

Scopus Document Identifier

  • 84987937789

Digital Object Identifier (DOI)

  • 10.1002/cmdc.201600301

PubMed ID

  • 27471800

Additional Document Info

volume

  • 11

issue

  • 18