PTEN regulates EG5 to control spindle architecture and chromosome congression during mitosis. Academic Article uri icon

Overview

abstract

  • Architectural integrity of the mitotic spindle is required for efficient chromosome congression and accurate chromosome segregation to ensure mitotic fidelity. Tumour suppressor PTEN has multiple functions in maintaining genome stability. Here we report an essential role of PTEN in mitosis through regulation of the mitotic kinesin motor EG5 for proper spindle architecture and chromosome congression. PTEN depletion results in chromosome misalignment in metaphase, often leading to catastrophic mitotic failure. In addition, metaphase cells lacking PTEN exhibit defects of spindle geometry, manifested prominently by shorter spindles. PTEN is associated and co-localized with EG5 during mitosis. PTEN deficiency induces aberrant EG5 phosphorylation and abrogates EG5 recruitment to the mitotic spindle apparatus, leading to spindle disorganization. These data demonstrate the functional interplay between PTEN and EG5 in controlling mitotic spindle structure and chromosome behaviour during mitosis. We propose that PTEN functions to equilibrate mitotic phosphorylation for proper spindle formation and faithful genomic transmission.

publication date

  • August 5, 2016

Research

keywords

  • Chromosome Segregation
  • Kinesins
  • Mitosis
  • PTEN Phosphohydrolase
  • Spindle Apparatus

Identity

PubMed Central ID

  • PMC4980451

Scopus Document Identifier

  • 84981273822

Digital Object Identifier (DOI)

  • 10.1038/ncomms12355

PubMed ID

  • 27492783

Additional Document Info

volume

  • 7