Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-parameter Flow Cytometry-Based Sorting. Academic Article uri icon

Overview

abstract

  • It is well established that tumors evolve together with nonmalignant cells, such as fibroblasts, endothelial cells, and immune cells. These cells constantly entangle and interact with each other creating the tumor microenvironment. Immune cells can exert both tumor-promoting and tumor-protective functions. Detailed phenotypic and functional characterization of intra-tumoral immune cell subsets has become increasingly important in the field of cancer biology and cancer immunology. In this chapter, we describe a method for isolation of viable and pure immune cell subsets from freshly isolated murine solid tumors and organs. First, we describe a protocol for the generation of single-cell suspensions from tumors and organs using mechanical and enzymatic strategies. In addition, we describe how immune cell subsets can be purified by consecutive magnetic cell sorting and multi-parameter flow cytometry-based cell sorting.

publication date

  • January 1, 2016

Research

keywords

  • Flow Cytometry
  • Immunomagnetic Separation
  • Neoplasms

Identity

Scopus Document Identifier

  • 85007471136

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-3801-8_10

PubMed ID

  • 27581019

Additional Document Info

volume

  • 1458