Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs. Academic Article uri icon

Overview

abstract

  • We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.

publication date

  • October 21, 2016

Research

keywords

  • Anal Canal
  • Enterobacteriaceae
  • Multiplex Polymerase Chain Reaction
  • beta-Lactamases

Identity

PubMed Central ID

  • PMC5075117

Scopus Document Identifier

  • 84994545642

Digital Object Identifier (DOI)

  • 10.1016/j.diagmicrobio.2015.03.011

PubMed ID

  • 27600053

Additional Document Info

volume

  • 60

issue

  • 11