Characterization of human and Staphylococcus aureus proteins in respiratory mucosa by in vivo- and immunoproteomics. Academic Article uri icon

Overview

abstract

  • UNLABELLED: Staphylococcus aureus is a Gram-positive opportunistic bacterium which can be found as a commensal in the nares of about 50% of the human population. Besides asymptomatic carriage, S. aureus has also been found to colonize nasal polyps, a subform of chronic rhinosinusitis, in 60 to 100% of cases, and even reside intracellularly in nasal polyp tissue. The aim of this study was to shed light on the behavior of S. aureus in the human airways by analyzing S. aureus-specific proteins in nasal polyp tissue from patients with chronic rhinosinusitis and to characterize the immunogenic potential of the identified (mainly secreted) proteins. As a result, in total >600 S. aureus proteins were identified by high resolution mass spectrometry or multiple reaction monitoring. Of those roughly 180 are typically localized in the membrane, surface exposed or secreted. For 115 S. aureus proteins, partially also detected in vivo by mass spectrometry, IgA- and IgG-specific antibody signals were profiled. Strong antibody signals were predominantly found for surface expose or secreted proteins. SIGNIFICANCE: In this study, we used high resolution mass spectrometry to identify S. aureus proteins directly in infected nasal polyp tissue. We discovered bacterial proteins involved in invasion of tissue, virulence, bacterial signal transduction or acquisition of nutrients. Some of the detected superantigens and Spls are known to provoke secretion of a broad spectrum of cytokines. Therefore, our manuscript contains new information about the invasion of S. aureus in nasal polyp tissue and its protein-specific immunogenicity.

authors

  • Schmidt, Frank
  • Meyer, Tanja
  • Sundaramoorthy, Nandakumar
  • Michalik, Stephan
  • Surmann, Kristin
  • Depke, Maren
  • Dhople, Vishnu
  • Gesell Salazar, Manuela
  • Holtappels, Gabriele
  • Zhang, Nan
  • Bröker, Barbara M
  • Bachert, Claus
  • Völker, Uwe

publication date

  • January 15, 2017

Research

keywords

  • Bacterial Proteins
  • Nasal Polyps
  • Proteomics
  • Respiratory Mucosa
  • Staphylococcus aureus

Identity

Scopus Document Identifier

  • 85010299064

Digital Object Identifier (DOI)

  • 10.1016/j.jprot.2017.01.008

PubMed ID

  • 28099884

Additional Document Info

volume

  • 155