Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination. Academic Article uri icon

Overview

abstract

  • Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen- or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750 × 750 ?? ? m 2 . We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2 × 2 ?? cm 2 of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant.

publication date

  • March 1, 2017

Research

keywords

  • Breast
  • Breast Diseases
  • Microscopy, Confocal

Identity

PubMed Central ID

  • PMC5361391

Scopus Document Identifier

  • 85016422321

Digital Object Identifier (DOI)

  • 10.1117/1.3149853

PubMed ID

  • 28327961

Additional Document Info

volume

  • 22

issue

  • 3