Inhibition of AKT1 signaling promotes invasion and metastasis of non-small cell lung cancer cells with K-RAS or EGFR mutations. Academic Article uri icon

Overview

abstract

  • Accumulating evidence supports a role of the PI3K-AKT pathway in the regulation of cell motility, invasion and metastasis. AKT activation is known to promote metastasis, however under certain circumstances, it also shows an inhibitory activity on metastatic processes, and the cause of such conflicting results is largely unclear. Here we found that AKT1 is an important regulator of metastasis and down-regulation of its activity is associated with increased metastatic potential of A549 cells. Inhibition of AKT1 enhanced migration and invasion in KRAS- or EGFR-mutant non-small cell lung cancer (NSCLC) cells. The allosteric AKT inhibitor MK-2206 promoted metastasis of KRAS-mutated A549 cells in vivo. We next identified that the phosphorylation of Myristoylated alanine-rich C-kinase substrate (MARCKS) and LAMC2 protein level were increased with AKT1 inhibition, and MARCKS or LAMC2 knockdown abrogated migration and invasion induced by AKT1 inhibition. This study unravels an anti-metastatic role of AKT1 in the NSCLC cells with KRAS or EGFR mutations, and establishes an AKT1-MARCKS-LAMC2 feedback loop in this regulation.

publication date

  • August 1, 2017

Research

keywords

  • Carcinoma, Non-Small-Cell Lung
  • Cell Movement
  • Laminin
  • Myristoylated Alanine-Rich C Kinase Substrate
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins p21(ras)

Identity

PubMed Central ID

  • PMC5539338

Scopus Document Identifier

  • 85026735200

Digital Object Identifier (DOI)

  • 10.1007/978-1-60327-216-2_14

PubMed ID

  • 28765579

Additional Document Info

volume

  • 7

issue

  • 1