Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue. Academic Article uri icon

Overview

abstract

  • Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovarian tissue that is co-transplanted with exogenous endothelial cells (ExEC). ExECs were capable of forming functionally perfused vessels at the host/graft interface and increased both viability and follicular volume in ExEC-assisted grafts with resumption of antral follicle development in long-term grafts. ExECs that were engineered to constitutively express anti-mullerian hormone (AMH) induced a greater proportion of quiescent primordial follicles than control ExECs, indicating suppression of premature mobilization that has been noted in the context of ovarian tissue transplantation. These findings present a cell-based strategy that combines accelerated perfusion with direct paracrine delivery of a bioactive payload to transplanted ovarian tissue.

publication date

  • August 15, 2017

Research

keywords

  • Endothelial Cells
  • Endothelium
  • Neovascularization, Physiologic
  • Ovary
  • Paracrine Communication
  • Tissue Engineering

Identity

PubMed Central ID

  • PMC5557862

Scopus Document Identifier

  • 85027527811

Digital Object Identifier (DOI)

  • 10.1210/edrv-17-2-121

PubMed ID

  • 28811567

Additional Document Info

volume

  • 7

issue

  • 1