Ion Binding to Transport Proteins using Isothermal Titration Calorimetry. Academic Article uri icon

Overview

abstract

  • Isothermal titration calorimetry (ITC) is an emerging, label-free technology used to measure ligand binding to membrane proteins. This technology utilizes a titration calorimeter to measure the heat exchange upon ligands binding to proteins, the magnitude of which is based on the overall enthalpy of the reaction. In this protocol, the steps we and others use to measure ion binding to ion transport proteins are described.

publication date

  • January 1, 2018

Research

keywords

  • Carrier Proteins
  • Ions

Identity

Scopus Document Identifier

  • 85032190074

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7362-0_22

PubMed ID

  • 29058200

Additional Document Info

volume

  • 1684