Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach. Academic Article uri icon

Overview

abstract

  • Antibodies that bind peptide-MHC (pMHC) complex in a manner akin to T-cell receptor (TCR) have not only helped in understanding the mechanism of TCR-pMHC interactions in the context of T-cell biology, but also spurred considerable interest in recent years as potential cancer therapeutics. Traditional methods to generate such antibodies using hybridoma and B-cell sorting technologies are sometimes inadequate, possibly due to the small contribution of peptide to the overall B-cell epitope space on the surface of the pMHC complex (typical peptide MW = 1 kDa versus MHC MW = 45 kDa), and to the multiple efficiency limiting steps inherent in these methods. In this chapter, we describe a phage display approach for the rapid generation of such antibodies with high specificity and affinity.

publication date

  • January 1, 2018

Research

keywords

  • Epitopes, B-Lymphocyte
  • Gene Library
  • Histocompatibility Antigens
  • Peptide Library

Identity

Scopus Document Identifier

  • 85033700140

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7447-4_13

PubMed ID

  • 29116509

Additional Document Info

volume

  • 1701