Overexpression of Map3k7 activates sinoatrial node-like differentiation in mouse ES-derived cardiomyocytes. Academic Article uri icon

Overview

abstract

  • In vivo, cardiomyocytes comprise a heterogeneous population of contractile cells defined by unique electrophysiologies, molecular markers and morphologies. The mechanisms directing myocardial cells to specific sub-lineages remain poorly understood. Here we report that overexpression of TGFβ-Activated Kinase (TAK1/Map3k7) in mouse embryonic stem (ES) cells faithfully directs myocardial differentiation of embryoid body (EB)-derived cardiac cells toward the sinoatrial node (SAN) lineage. Most cardiac cells in Map3k7-overexpressing EBs adopt markers, cellular morphologies, and electrophysiological behaviors characteristic of the SAN. These data, in addition to the fact that Map3k7 is upregulated in the sinus venous-the source of cells for the SAN-suggest that Map3k7 may be an endogenous regulator of the SAN fate.

publication date

  • December 27, 2017

Research

keywords

  • Cell Differentiation
  • MAP Kinase Kinase Kinases
  • Myocytes, Cardiac
  • Sinoatrial Node

Identity

PubMed Central ID

  • PMC5744947

Scopus Document Identifier

  • 85039723134

Digital Object Identifier (DOI)

  • 10.3727/096368911X565038

PubMed ID

  • 29281682

Additional Document Info

volume

  • 12

issue

  • 12