Mononuclear phagocytes as targets for cytolytic T lymphocytes. Academic Article uri icon

Overview

abstract

  • Mouse peritoneal macrophages and human blood monocytes are excellent targets for the assay of cytolytic T lymphocytes that develop in the murine and human mixed leukocyte reactions, respectively. 51Cr release in a standard 4 h assay is equivalent to the best release that is obtained with conA-induced T lymphoblasts. Spontaneous release is consistently less than 20% of the total cell label, and syngeneic macrophages are not lysed. Unlike lymphoblasts, mononuclear phagocytes can be used either as freshly isolated cells or after prolonged periods (1-7 days) in culture.

publication date

  • June 26, 1987

Research

keywords

  • Phagocytes
  • T-Lymphocytes, Cytotoxic

Identity

Scopus Document Identifier

  • 0023196571

Digital Object Identifier (DOI)

  • 10.1016/0022-1759(87)90177-3

PubMed ID

  • 2955054

Additional Document Info

volume

  • 100

issue

  • 1-2