Accessory cell requirements for the mixed-leukocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells. Academic Article uri icon

Overview

abstract

  • Human blood dendritic cells can be enriched to 40-80% purity by a new technique that is simpler, provides greater yields than prior methods, and resolves other populations that are enriched in monocytes and B and T lymphocytes. The procedure involves separation over two Percoll gradients after 0 and 2 days of culture, followed by removal of contaminating monocytes by panning on plates coated with human Ig. The resultant dendritic cell-enriched fraction is 10 times or more potent than the monocyte-enriched populations in stimulating T-cell proliferative responses to alloantigens and to Con A. Small B lymphocytes are inactive in both systems. Dendritic cells do not initiate mitogenesis to anti-CD3 monoclonal antibodies, a response for which the monocyte appears to be the critical accessory cell.

publication date

  • January 1, 1988

Research

keywords

  • Antigen-Presenting Cells
  • Dendritic Cells

Identity

Scopus Document Identifier

  • 0023870862

Digital Object Identifier (DOI)

  • 10.1016/0008-8749(88)90061-5

PubMed ID

  • 2962741

Additional Document Info

volume

  • 111

issue

  • 1